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DNA-only Transposons02:57

DNA-only Transposons

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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Related Experiment Video

Updated: Apr 26, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

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A dual input DNA-based molecular switch.

Irina V Nesterova1, Siddieg O Elsiddieg, Evgueni E Nesterov

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA. inester@lsu.edu.

Molecular Biosystems
|August 8, 2014
PubMed
Summary

Scientists created a novel DNA molecular switch. This switch detects pH changes and enzyme activity to produce a synthesized DNA output, enabling new biological applications.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Developing responsive molecular systems is crucial for biological sensing.
  • Existing systems often lack simplicity, tunability, or intracellular compatibility.

Purpose of the Study:

  • To design and characterize a DNA-based molecular switch.
  • To enable sensing of physiological pH and enzymatic activity.
  • To generate an in situ synthesized oligonucleotide output.

Main Methods:

  • Utilized allosteric interactions between i-motif and hairpin stem structures in DNA.
  • Engineered dual input sensing domains for pH and enzymatic activity.
  • Characterized molecular switch performance and intracellular compatibility.

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Main Results:

  • Successfully designed and validated a DNA molecular switch.
  • Demonstrated orthogonal sensing of pH (alkalinization) and enzymatic activity.
  • Achieved in situ synthesis of oligonucleotide outputs.

Conclusions:

  • The developed DNA switch offers molecular simplicity and tunability.
  • It exhibits orthogonality in its sensing domains.
  • The switch is compatible with intracellular operation and delivery, paving the way for advanced biosensors.