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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Related Experiment Video

Updated: Apr 26, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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Generation of physical map contig-specific sequences.

Yanliang Jiang1, Peng Xu1, Zhanjiang Liu2

  • 1Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences Beijing, China.

Frontiers in Genetics
|August 8, 2014
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing advances genome assembly, but contigs and scaffolds can be too small for genetic analysis. Physical map-derived sequences improve whole genome assembly quality, especially scaffolding.

Keywords:
BAC end sequencesassemblyphysical map contig-specific sequencesscaffoldingwhole genome sequencing

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Next-generation sequencing (NGS) enables whole genome sequencing across diverse species.
  • Current NGS technologies often yield genome assemblies with limitations in accuracy, contiguity, connectivity, and completeness.
  • Small contigs and scaffolds hinder the utility of whole genome sequences for genetic analyses.

Purpose of the Study:

  • To review the principles, procedures, and applications of physical map-derived sequences.
  • To highlight the importance of these sequences for enhancing genome assembly quality, particularly scaffolding.
  • To focus on physical map contig-specific sequences for improved genome assembly.

Main Methods:

  • Review of existing literature on physical mapping techniques.
  • Analysis of data from physical map contig-specific sequences.
  • Discussion of scaffolding strategies utilizing physical map information.

Main Results:

  • Physical map-derived sequences provide crucial information for genome scaffolding.
  • These sequences significantly enhance the contiguity and connectivity of genome assemblies.
  • Physical map contig-specific sequences offer a powerful resource for resolving complex genomic regions.

Conclusions:

  • Physical map-derived sequences are essential for improving whole genome assembly quality.
  • Utilizing these sequences overcomes limitations in current NGS assembly methods.
  • The application of physical map contig-specific sequences is key to advancing genetic analysis through better genome assemblies.