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Validation of high throughput sequencing and microbial forensics applications.

Bruce Budowle1, Nancy D Connell2, Anna Bielecka-Oder3

  • 1Department of Molecular and Medical Genetics, Institute of Applied Genetics, University of North Texas Health Science Center, Fort Worth, Texas, USA ; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia.

Investigative Genetics
|August 8, 2014
PubMed
Summary

High throughput sequencing (HTS) offers rapid microbial genomics for biocrime investigations. Validating HTS methods is crucial for accurate microbial forensics and national security.

Keywords:
BioinformaticsHigh throughput sequencingLibrary preparationMicrobial forensicsSample preparationValidation

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Area of Science:

  • Microbial Forensics
  • Genomics
  • Biosecurity

Background:

  • High throughput sequencing (HTS) generates extensive, high-quality data for microbial genomics.
  • HTS accelerates evidence collection and characterization in microbial forensics, aiding biocrime and bioterrorism investigations.

Purpose of the Study:

  • To present criteria for validating High throughput sequencing (HTS) systems in microbial forensics.
  • To establish guiding principles for HTS method validation to ensure accurate and credible results.

Main Methods:

  • The study outlines validation criteria for HTS, focusing on sample preparation, sequencing, and data analysis.
  • It emphasizes defining HTS utility within established operating conditions and tolerances.

Main Results:

  • Validation criteria are presented for HTS application in microbial forensics.
  • The criteria provide a foundation for establishing and implementing HTS tools.

Conclusions:

  • Validated HTS methods are essential for reliable microbial forensics, supporting investigative leads and attribution.
  • Implementing validated HTS enhances public safety and national security by providing powerful tools for biocrime investigations.