Related Experiment Video
Updated: Apr 26, 2026
09:49
Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
Published on: July 14, 2014
9.7K
Biosynthesis of silver nanoparticles using Saccharomyces cerevisiae
Hassan Korbekandi1, Soudabeh Mohseni2, Rasoul Mardani Jouneghani2
1a Genetics and Molecular Biology Department, School of Medicine , Isfahan University of Medical Sciences , Isfahan , Iran.
Artificial Cells, Nanomedicine, and Biotechnology
|August 8, 2014
Summary
Freshly cultured Saccharomyces cerevisiae efficiently biosynthesized silver nanoparticles (NPs), which were spherical, 2-20 nm in size, and located inside and outside yeast cells.
Area of Science:
- Biotechnology and Nanomaterials Science
Background:
- Biosynthesis of nanoparticles offers a sustainable alternative to chemical methods.
- Saccharomyces cerevisiae is a well-characterized yeast with potential for biotransformation applications.
Purpose of the Study:
- To investigate the biosynthesis of silver nanoparticles (NPs) using Saccharomyces cerevisiae.
- To characterize the size, shape, and location of biosynthesized silver NPs.
Main Methods:
- Utilizing both dried and freshly cultured Saccharomyces cerevisiae as biocatalysts for silver NP synthesis.
- Analyzing the synthesized NPs for size and shape using appropriate characterization techniques.
Main Results:
- Freshly cultured Saccharomyces cerevisiae demonstrated significantly higher production of silver NPs compared to dried yeast.
- The biosynthesized silver NPs were predominantly spherical, with sizes ranging from 2-20 nm.
- The most frequent NP size observed was 5.4 nm.
- Silver NPs were localized intracellularly, within the cell membrane, and extracellularly, including during exocytosis.
Conclusions:
- Freshly cultured Saccharomyces cerevisiae is an effective biocatalyst for the efficient biosynthesis of silver nanoparticles.
- The study provides insights into the intracellular and extracellular mechanisms of silver NP formation and release by yeast.
Related Concept Videos
01:22Bioreactor Controls-III
67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
01:25Microbes in Beverage Production
296
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
296