In vitro approaches to assessing the toxicity of quantum dots

Advances in nanotechnology have produced a new class of fluorescent nanoparticles known as quantum dots (Qdots). Compared with organic dyes and fluorescent proteins, Qdots offer several unique advantages in terms of spectral range, brightness, and photostability. Relative to other imaging modalities, optical imaging with Qdots is highly sensitive, quantitative, and capable of multiplexing. Thus, Qdots are being developed for a wide range of applications, including biomedical imaging. Qdot production has also emerged in a number of industrial applications, such as optoelectronic devices and photovoltaic cells. This widespread development and use of Qdots has outpaced research progress on their potential cytotoxicity, engendering major concerns surrounding occupational, environmental, and diagnostic exposures. Given the extensive physicochemical heterogeneity of Qdots (size, charge, chemical composition, solubility, etc.), high-throughput in vitro cytotoxicity assays represent a feasible means of determining effects of multiple variables and can inform design of lower-throughput in vivo cytotoxicity studies. Here, we describe the application of two commonly used assays, lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS), for detection of Qdot-induced cytotoxicity.