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A simple and fast method for iron determination with ferrozine after proteolytic disruption of iron-transfer in
Abstract:
A direct method for iron determination with ferrozine in blood serum is described. Iron is liberated and proteins degraded by pepsin in hydrochloric acid medium. At appropriate pH the iron-ferrozine complex forms and stabilizes in five minutes. Accuracy of the method is 99.3-101.4%, precision within run and day to day 0.73 and 1.1% respectively, and linearity till 72 mumol/L iron.