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Related Experiment Video

Updated: Apr 26, 2026

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
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Using the cytosensor microphysiometer to assess ocular toxicity.

Jennifer R Nash1, Greg Mun, Hans A Raabe

  • 1Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.

Current Protocols in Toxicology
|August 9, 2014
PubMed
Summary

The Cytosensor Microphysiometer assay measures in vitro cytotoxicity by detecting reduced cellular metabolic rates in L929 cells exposed to chemicals. This method quanties chemical-induced eye damage by determining the metabolic rate decline of 50% (MRD50).

Keywords:
Cytosensor Microphysiometereye irritationin vitro cytotoxicitymetabolic rateocular irritation

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Area of Science:

  • Toxicology
  • Cell Biology
  • Biomedical Engineering

Background:

  • In vitro cytotoxicity assays are crucial for estimating chemical-induced eye damage.
  • The Cytosensor Microphysiometer offers a method to evaluate cytotoxicity by assessing cellular metabolic activity.

Purpose of the Study:

  • To evaluate the utility of the Cytosensor Microphysiometer for measuring in vitro cytotoxicity.
  • To determine the metabolic rate decline of 50% (MRD50) as a key endpoint for chemical exposure.

Main Methods:

  • Utilized L929 cells and the Cytosensor Microphysiometer to measure extracellular acidification rates.
  • Assessed the reduction in metabolic rate as an indicator of cytotoxicity following exposure to test materials.
  • Quantified changes in pH to estimate metabolic rate and determine the MRD50 value.

Main Results:

  • The Cytosensor Microphysiometer successfully measured test material-induced reductions in cellular metabolic rates.
  • A decrease in acid byproduct release correlated with increasing concentrations of cytotoxic materials, indicating cell death.
  • The metabolic rate decline of 50% (MRD50) was established as a quantifiable endpoint.

Conclusions:

  • The Cytosensor Microphysiometer is an effective tool for in vitro cytotoxicity assessment.
  • This method provides a reliable estimation of chemical-induced cellular damage relevant to eye safety evaluations.
  • The MRD50 value serves as a valuable metric for quantifying cytotoxic effects.