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Updated: Apr 26, 2026
Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model
Published on: May 6, 2020
[Osteochodral lesion mouse model: an alternative for experimental work]
R Martinez1, D Figueroa1, R Calvo1
1Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
Objective:
To report a reproducible and inexpensive model of critical osteochondral lesion (LOC) in adult mice for experimental studies
Material And Method:
An experimental study was conducted on 20 BKS mice of 15 weeks old, in which a LOC of 0.5mm in diameter was made in the trochlear groove. Ten animals were sacrificed at day 7, and the other 10 animals at day 14 of follow up. To assess the ability of the animal to repair/regenerate, a histological analysis was performed using hematoxylin-eosin and safranin-O stains, and the results were evaluated by the ICRS scale using areas of healthy cartilage from the same joint as control. The Mann-Whitney U test was used for the statistical analyses of scores (averages).
Results:
Significant differences were found in days 7 and 14 between the LOC area and control areas, but no differences were found between the day 7 and day 14.
Conclusion:
This model of LOC in the trochlear groove of adult mice is highly reproducible, and could be used in further studies to obtain better treatments for chondral pathologies.
