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Impact of placental growth factor deficiency on early mouse implant site angiogenesis

M T Rätsep1, P Carmeliet2, M A Adams1

  • 1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.

Placenta
|August 10, 2014
PubMed

Effects of placental growth factor (PGF), an angiokine product of fetal trophoblasts and maternal decidual cells, on early decidual angiogenesis are undefined. We used whole-mount immunofluorescence analyses to compare uterus and gestation day 4.5-9.5 mouse implantation sites that differed genetically in fetal or maternal PGF deficiency. Implant site number and embryonic development were similar in Pgf(-/-) and Pgf(+/+) females although Pgf(-/-) lymphatic vessels were anomalous. Correct, fine branching angiogenesis of anti-mesometrial vessels required both conceptus and maternal PGF; correct mesometrial branching angiogenesis depended solely upon conceptus PGF. Thus, PGF is non-redundant for optimizing branching angiogenesis in early decidua.

Keywords:
DeciduaMouse pregnancyUltrastructureVascular endothelial growth factorWhole mount in situ immunohistochemistry

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