Related Experiment Videos
Penicillinase-producing Neisseria gonorrhoeae in Dhaka, Bangladesh
Abstract:
Among the 52 strains of Neisseria gonorrhoeae isolated from cases of acute urethritis attending the dermatovenerology department of different hospitals of Dhaka city, 5(9.6%) strains were identified as penicillinase-producing (PPNG) and had minimum inhibitory concentration (MIC) of penicillin 4.0 micrograms/ml or more. Of the rest 47 (90.4%) non-penicillinase-producing strains (non-PPNG), 18(38.3%) were fully sensitive to penicillin (MIC = 0.06 micrograms/ml or less), 25(53.2%) had diminished sensitivity (MIC = 0.125 to 0.5 micrograms/ml) and 4(8.5%) were resistant to penicillin (3 had MIC 1 micrograms/ml and 1 had MIC 2 micrograms/ml).
Insights
Penicillinase-producing Neisseria gonorrhoeae (PPNG) strains were identified in 9.6% of acute urethritis cases in Dhaka. Most non-penicillinase-producing strains showed reduced sensitivity or resistance to penicillin.
Area of Science:
- Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Neisseria gonorrhoeae is a major cause of sexually transmitted infections.
- Penicillin resistance in Neisseria gonorrhoeae is a growing global health concern.
- Understanding local resistance patterns is crucial for effective treatment strategies.
Purpose of the Study:
- To determine the prevalence of penicillinase-producing Neisseria gonorrhoeae (PPNG) and non-penicillinase-producing Neisseria gonorrhoeae (non-PPNG) strains.
- To assess the antimicrobial susceptibility of Neisseria gonorrhoeae isolates to penicillin in Dhaka city.
- To evaluate the minimum inhibitory concentrations (MICs) of penicillin against different Neisseria gonorrhoeae phenotypes.
Main Methods:
- Isolation and identification of Neisseria gonorrhoeae strains from patients with acute urethritis.
- Detection of penicillinase production in Neisseria gonorrhoeae isolates.
- Determination of minimum inhibitory concentrations (MICs) of penicillin using standard microbiological techniques.
Main Results:
- Out of 52 Neisseria gonorrhoeae strains, 5 (9.6%) were identified as penicillinase-producing (PPNG) with high penicillin MICs (≥ 4.0 µg/ml).
- Among the 47 non-penicillinase-producing strains (non-PPNG), 38.3% were fully sensitive to penicillin (MIC ≤ 0.06 µg/ml).
- A significant proportion of non-PPNG strains exhibited diminished sensitivity (53.2%, MIC 0.125-0.5 µg/ml) or resistance (8.5%, MIC 1-2 µg/ml) to penicillin.
Conclusions:
- Penicillinase-producing Neisseria gonorrhoeae (PPNG) strains represent a notable proportion of infections in Dhaka.
- A substantial percentage of non-penicillinase-producing strains also display reduced susceptibility or resistance to penicillin.
- These findings highlight the challenges in treating gonococcal infections with penicillin and underscore the need for continuous antimicrobial surveillance.