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In situ hybridization using biotin-labeled oligonucleotides: probe labeling and procedures for mRNA detection
1Research Center for Medical Biotechnology, State Serum Institute, Copenhagen, Denmark.
Archives of Histology and Cytology
|January 1, 1989
Abstract:
Methods for non-radioactive in situ hybridization are rapidly progressing in refinement and application. In this overview, we present our experiences with the use of synthetic oligodeoxynucleotides, labeled with biotin- or digoxigenin-tagged nucleotides by the terminal transferase method.