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Glycoprotein profiling of stem cells using lectin microarray based on surface plasmon resonance imaging.

Amita Nand1, Vikramjeet Singh1, Peizhe Wang2

  • 1National Center for Nanoscience and Technology, Beijing 100190, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.

Analytical Biochemistry
|August 10, 2014
PubMed
Summary

This study introduces a novel lectin microarray method combined with surface plasmon resonance imaging (SPRi) for rapid, label-free stem cell glycan analysis. This technique accurately distinguishes pluripotent stem cells, aiding cell therapy quality control.

Keywords:
GlycoproteinLectin microarrayPluripotencySPRiStem cell

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Area of Science:

  • Glycomics
  • Biotechnology
  • Stem Cell Biology

Background:

  • Lectin microarrays offer a new approach for glycan analysis.
  • Characterizing stem cell surface glycans is crucial for regenerative medicine and cell therapy.

Purpose of the Study:

  • To develop a rapid, label-free method for stem cell glycan profiling using lectin microarrays and SPRi.
  • To distinguish between pluripotent and non-pluripotent mouse stem cells based on their glycan profiles.

Main Methods:

  • Utilized a lectin microarray platform with 40 lectins across seven glyco-binding motifs.
  • Employed surface plasmon resonance imaging (SPRi) for label-free detection.
  • Analyzed three mouse cell lines: embryonic stem cells (mESCs), induced pluripotent stem cells (miPSCs), and embryonic fibroblasts (MEFs).

Main Results:

  • Successfully differentiated pluripotent mouse stem cells (mESCs, miPSCs) from non-pluripotent cells (MEFs).
  • Identified eight key lectins (DBA, MAL, PHA_E, PHA_L, EEL, AAL, PNA, SNA) that effectively define pluripotency.
  • Developed a discriminant function based on lectin reactivities with high accuracy for determining stem cell pluripotency.

Conclusions:

  • Glycomic analysis using lectin microarrays and SPRi provides a powerful tool for stem cell characterization.
  • This approach offers a novel and comprehensive strategy for quality control in cell-based therapies and regenerative medicine.