[Mesenchymal stem cells promote mouse breast tumor progression by inducing the suppressive function of
Xiaoyu Hu1, Yuechen Luo1, Yushan Zhou1
1State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
Objective:
To investigate the role of mesenchymal stem cells (MSCs) in tumor progression by inducing immuno-suppressive function of myeloid-derived CD11b⁺ Gr1⁺ cells (BM-CD11b⁺ Gr1⁺ cells).
Methods:
After co-cultured with MSCs, the immunophenotypes of BM-CD11b⁺ Gr1⁺ cells were tested by flow cytometry. The influence of MSCs on CD11b⁺ Gr1⁺ cells was evaluated by T cell proliferation assay after cultured with or without MSCs. The mouse 4T1 breast tumor model was established to explore the effect on tumor growth.
Results:
MSCs promoted the survival of CD11b⁺ Gr1⁺ cells and induced the generation of CD11b⁺ Gr1⁺ cells from CD11b⁻ Gr1⁻ cells sorted from bone marrow. MSCs also enhanced the ability of BM-CD11b⁺ Gr1⁺ cells to suppress T cell proliferation and activation. CD11b⁺ Gr1⁺ cells after co-cultured with MSCs promoted 4T1 tumor growth and accelerated death of tumor-bearing mice.
Conclusion:
MSCs can promote tumor progression by inducing suppressive function of myeloid-derived CD11b⁺ Gr1⁺ cells from bone marrow.
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