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Updated: Apr 26, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
An in silico model for rapid identification of multiple bacteria in resource limited laboratories
Subramanian Babu1, Asit Ranjan Ghosh, Ramamoorthy Siva
1School of Bio Sciences and Technology, VIT University, Vellore, 632014, India, babu.s@vit.ac.in.
Abstract:
We have developed an in silico model for simplified detection of five major food borne bacteria using common PCR primer based on conserved region flanking a variable region in the first rRNA operon sequences. The RFLP pattern exhibited on virtual SmaI and MboI digestion were unique to each genus. This design is based on the combination of comparative sequence analysis, conventional PCR and restriction digestion methods. We believe this approach as a better alternative to 16S rRNA sequencing based identification / detection of bacteria and suited for the resource limited academic research laboratories in which variety of bacteria are used in different students' projects.
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