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Updated: Apr 26, 2026

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
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Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

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Adaptive protein and phosphoprotein networks which promote therapeutic sensitivity or acquired resistance

John Haley, Forest M White1

  • 1‡Department of Biological Engineering and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A.

Biochemical Society Transactions
|August 12, 2014
PubMed

Insights

Mass spectrometry-based phosphoproteomics can identify cancer signaling networks driving tumor growth and resistance. This approach aids in developing targeted therapies to overcome treatment failure.

Area of Science:

  • Oncology
  • Proteomics
  • Molecular Biology

Background:

  • Cancer therapies often fail due to therapeutic resistance, leading to tumor progression and recurrence.
  • Existing molecularly targeted therapies face limitations in overcoming resistance mechanisms.

Purpose of the Study:

  • To review the application of mass spectrometry-based phosphoproteomics in quantifying oncogenic signaling networks.
  • To highlight the role of phosphoproteomics in understanding tumor growth, invasion, and survival under therapy.

Main Methods:

  • Utilizing mass spectrometry (MS)-based phosphoproteomics to quantitatively profile protein phosphorylation.
  • Analyzing signaling networks associated with tumor development and therapeutic resistance.

Main Results:

  • MS-based phosphoproteomics can identify key signaling pathways driving tumor progression and invasion.
  • This technique reveals networks crucial for tumor cell survival during chemotherapy.

Conclusions:

  • Quantitative protein phosphorylation profiling is essential for designing effective cancer treatments.
  • Targeting both initial oncogenic drivers and resistance mechanisms simultaneously is crucial for therapeutic success.

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