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The basic region of Fos mediates specific DNA binding.

Y Nakabeppu1, D Nathans

  • 1Howard Hughes Medical Institute, Baltimore, MD.

The EMBO Journal
|December 1, 1989
PubMed
Summary

The Fos and Jun basic regions of proteins contribute equally to DNA binding specificity in Fos-Jun heterodimers. This finding supports a model of their interaction with DNA half sites.

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Area of Science:

  • Molecular Biology
  • Protein-DNA Interactions
  • Transcription Factors

Background:

  • Fos and Jun proteins form heterodimers that bind to specific DNA sequences like the AP-1 site and CRE.
  • The DNA-binding domain comprises a basic region for DNA contact and a leucine repeat for dimerization.

Purpose of the Study:

  • To investigate if the Fos basic region contributes DNA-binding properties equivalent to the Jun basic region within Fos-Jun heterodimers.
  • To elucidate the roles of individual basic regions in sequence-specific DNA binding.

Main Methods:

  • Construction and analysis of Fos-Jun chimeric proteins with swapped basic regions and leucine repeats.
  • Assessment of heterodimer binding affinity and DNA sequence specificity to AP-1 and CRE sites.

Main Results:

  • Fos-Jun heterodimers, regardless of the origin of their basic regions (Fos or Jun), exhibited high-affinity binding to AP-1 and CRE.
  • Heterodimers with two Fos basic regions showed slightly higher affinity for AP-1 and CRE compared to those with two Jun basic regions.
  • DNA sequence specificity and contact sites were similar across different heterodimer configurations.

Conclusions:

  • The basic region of Fos confers DNA-binding properties equivalent to that of Jun in Fos-Jun heterodimers.
  • Both Fos and Jun basic regions interact with symmetrical DNA half sites within the target sequences.

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