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Updated: Apr 26, 2026
Reconstitution of Msp1 Extraction Activity with Fully Purified Components
Published on: August 10, 2021
mRNA transport meets membrane traffic
Ralf-Peter Jansen1, Dierk Niessing2, Sebastian Baumann3
1Eberhard Karls Universität Tübingen, Interfaculty Institute of Biochemistry, Hoppe-Seyler-Strasse 4, 72076 Tübingen, Germany.
Abstract:
Active transport and local translation of mRNAs ensure the appropriate spatial organization of proteins within cells. Recent work has shown that this process is intricately connected to membrane trafficking. Here, we focus on new findings obtained in fungal model systems. Important highlights are that RNA-binding proteins recognize cargo mRNA synergistically and that mRNAs are co-transported with membranous compartments such as the endoplasmic reticulum (ER) and endosomes. We further discuss a novel concept of endosome-coupled translation that loads shuttling endosomes with septin cargo, a process important for correct septin filamentation. Interestingly, evidence is accumulating that RNA and membrane trafficking are also tightly interwoven in higher eukaryotes, suggesting that this phenomenon is a common theme and not an exception restricted to fungi.
Insights
Messenger RNAs (mRNAs) are transported with cellular compartments like the endoplasmic reticulum and endosomes, guided by RNA-binding proteins. This co-transport is crucial for protein localization and function, even in higher eukaryotes.
Area of Science:
- Cell Biology
- Molecular Biology
- Mycology
Background:
- Cellular protein localization relies on active mRNA transport and local translation.
- Emerging evidence links mRNA transport and local translation to membrane trafficking pathways.
Purpose of the Study:
- To investigate the interplay between RNA transport and membrane trafficking in fungal model systems.
- To highlight novel findings on mRNA recognition, co-transport with organelles, and endosome-coupled translation.
Main Methods:
- Studies conducted in fungal model systems.
- Analysis of RNA-binding protein interactions with mRNA.
- Observation of mRNA co-transport with endoplasmic reticulum and endosomes.
Main Results:
- RNA-binding proteins recognize and bind to cargo mRNAs synergistically.
- mRNAs are actively co-transported with membranous cellular compartments, including the endoplasmic reticulum and endosomes.
- A novel mechanism of endosome-coupled translation was identified, essential for septin filamentation.
Conclusions:
- RNA and membrane trafficking are tightly interconnected processes.
- This interplay is vital for spatial protein organization and cellular function.
- Evidence suggests this phenomenon is conserved across eukaryotes, not limited to fungi.
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