Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell proliferation and oxidative stress.

R H Burdon1, V Gill, C Rice-Evans

  • 1Department of Bioscience and Biotechnology, Todd Centre, University of Strathclyde, Glasgow, UK.

Free Radical Research Communications
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dabrafenib and Trametinib Therapy for Advanced Anaplastic Thyroid Cancer - Real-World Outcomes From UK Centres.

Clinical oncology (Royal College of Radiologists (Great Britain))·2022
Same author

Differentiated Thyroid Cancer in Children: A UK Multicentre Review and Review of the Literature.

Clinical oncology (Royal College of Radiologists (Great Britain))·2019
Same author

Experiences of discrimination among people using mental health services in England 2008-2011.

The British journal of psychiatry. Supplement·2013
Same author

Preliminary evidence for biologic activity of toceranib phosphate (Palladia(®)) in solid tumours.

Veterinary and comparative oncology·2012
Same author

Thyroxine dosing after I131Radioiodine ablation for differentiated thyroid cancer.

Clinical oncology (Royal College of Radiologists (Great Britain))·2011
Same author

Reply to the editorial on the HiLo trial.

Clinical oncology (Royal College of Radiologists (Great Britain))·2008

Antioxidants like mannitol promote cell growth, potentially by protecting cellular mechanisms. However, oxidants can inhibit growth, though mild stress or low oxidant concentrations may paradoxically stimulate it.

Area of Science:

  • Cell Biology
  • Biochemistry

Background:

  • Cellular growth is influenced by oxidative stress.
  • Antioxidants and oxidants play critical roles in cell regulation.

Purpose of the Study:

  • To investigate the effects of antioxidants and oxidants on BHK-21 cell growth.
  • To explore the mechanisms underlying oxidant-induced growth inhibition and stimulation.

Main Methods:

  • Treatment of polyoma virus transformed and non-transformed BHK-21 cells with antioxidants (mannitol, butylated hydroxytoluene, alpha-tocopherol) and oxidants (hydrogen peroxide, t-butyl hydroperoxide).
  • Assessment of cell growth and overall cellular protein synthesis.
  • Evaluation of the role of radicals and 'prestressing' in cellular responses.

Main Results:

Related Experiment Videos

  • Antioxidants significantly enhanced BHK-21 cell growth, even without calf serum.
  • Oxidants inhibited cell growth and protein synthesis, likely via radical mechanisms.
  • Prestressing cells with heat or H2O2 reduced oxidant-induced inhibition.
  • Very low oxidant concentrations paradoxically stimulated cell growth, an effect enhanced by mannitol or DMSO, suggesting a non-radical mechanism.

Conclusions:

  • Antioxidants can protect and promote cell growth, possibly by preserving cellular control mechanisms.
  • Oxidative stress can inhibit cell growth, but cells can adapt through prestressing.
  • Low-dose oxidants may stimulate cell growth via non-radical pathways, modulated by other compounds.