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31P-NMR spectroscopy of phosphate compartmentation during ischaemia in hearts protected by cardioplegic treatment
A Stier1, S A Finch, H Schäfer
1Max-Planck-Institut für Biophysikalische Chemie, Göttingen, W. Germany.
Free Radical Research Communications
|January 1, 1989
Abstract:
Four tissue compartments, differing in proton and inorganic phosphate concentration, were resolved by 31P-NMR spectroscopy in samples from dog hearts after cardioplegic treatment with HTK solution. Inversion of the physiological cytoplasmic-mitochondrial pH gradient was observed. The considerable ensuing acidosis of the matrix is discussed with regard to a possible delocalization of ferrous ions.