Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reconstruction of septin higher-order nano-size structures in ovarian cancer cells uncover susceptibility to the septin-targeting small molecule UR214-9.

bioRxiv : the preprint server for biology·2026

Same author

Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes.

Nano letters·2026

Same author

T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signalling.

The EMBO journal·2026

Same author

Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stress.

bioRxiv : the preprint server for biology·2026

Same author

Local PI(4,5)P<sub>2</sub> synthesis by septin-associated PIPKIγ isoforms controls centralspindlin association with the midbody during cytokinesis.

Nature communications·2026

Same author

CDC42-Effector Proteins Regulate Higher Order Structure of Septins Required for CNS Myelin Integrity.

Glia·2026

Same journal

Spectroscopic Investigation of the In Vivo Light-Dependent Photodynamics of the Marine Diatom Phaeodactylum tricornutum.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

Same journal

Atomistic Insights into the Thermal Decomposition and Runaway Mechanism of Peroxypropionic Acid.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

Same journal

Hydrazine Adsorption on Hexagonal Ice (0001): First-Principles Investigations on Stability, Dynamics, and Chirality Changes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

Same journal

Sustainable Ball Milling-Assisted Synthesis of Bread Waste-Derived Highly Porous Carbons for Adsorption-Based Applications.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

Same journal

RNALig: An ML-Driven Structure-Based Scoring Function for Estimating Binding Affinities of RNA-Ligand Complexes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

Same journal

Photoswitchable Polar Azobenzene-Based Liquid Crystals for Electro-Optic and Optical Data Storage Applications.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 26, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Single-molecule localization microscopy using mCherry.

Christian M Winterflood¹, Helge Ewers

¹Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL (UK); Institute of Biochemistry, ETH Zürich, Otto-Stern-Weg 3, 8093 Zürich (Switzerland). christian.winterflood@kcl.ac.uk.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry

|August 12, 2014

Summary

This summary is machine-generated.

The red fluorescent protein mCherry can be used for super-resolution microscopy, achieving resolutions below 40 nm. This widely available protein enables advanced imaging without needing new constructs or external labeling.

Keywords:

fluorescent labeling mCherry nuclear pore protein structures super-resolution imaging

More Related Videos

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Related Experiment Videos

Last Updated: Apr 26, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Super-resolution microscopy requires specific fluorescent probes.
Existing fluorescent proteins may have limitations for advanced imaging techniques.
mCherry is a widely used red fluorescent protein in biological research.

Purpose of the Study:

To evaluate the suitability of mCherry for single-molecule super-resolution imaging.
To demonstrate the capability of mCherry in resolving subcellular structures with high resolution.
To assess the potential of mCherry for imaging challenging cellular targets.

Main Methods:

Utilizing mCherry's light-induced dark state and recovery properties.
Applying single-molecule super-resolution imaging techniques.
Imaging subcellular structures like microtubules and the nuclear pore complex.

Main Results:

Achieved super-resolution imaging with resolutions below 40 nm using mCherry.
Successfully imaged the C-terminus of the nuclear pore protein POM121.
Demonstrated comparable photon yield to advanced optical highlighter proteins.

Conclusions:

mCherry is a viable and effective fluorescent protein for super-resolution microscopy.
Existing mCherry fusion proteins can be readily used for super-resolution imaging.
Eliminates the need for developing new protein fusions or external labeling for super-resolution applications.