Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Altered Busulphan Exposure Associated with Concomitant Omeprazole during Hematopoietic Stem Cell Transplantation Conditioning: A Two-Case Report.

Clinical drug investigation·2026
Same author

Impact of Graft-Versus-Host Disease Prophylaxis on Long-Term Outcomes in Patients Surviving Beyond Two Years After Allogeneic Transplantation.

JCO oncology practice·2026
Same author

International Survey and Next-Generation Sequencing-Based Indel Chimerism Detection of Human Leukocyte Antigens Loss in Relapsed Haploidentical and Mismatched Unrelated Donor Transplant Recipients.

Transplantation and cellular therapy·2026
Same author

Reconsidering the role of bone marrow as a graft source in haploidentical allogeneic hematopoietic cell transplantation.

Bone marrow transplantation·2026
Same author

Toward a Standardized Definition of Reduced-Dose Post-transplant Cyclophosphamide in Allogeneic Hematopoietic Cell Transplantation.

Transplantation and cellular therapy·2026
Same author

Prior Malignancy History and Risk of Second Malignancies After Allogeneic Hematopoietic Cell Transplantation.

Transplantation and cellular therapy·2026

Related Experiment Video

Updated: Apr 26, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
07:16

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

13.8K

Novel method to characterize immune cells from human prostate tissue.

Melissa M Norström1, Emelie Rådestad, Arwen Stikvoort

  • 1Centre for Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital Huddinge, Stockholm, Sweden; Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden.

The Prostate
|August 12, 2014
PubMed
Summary

A new method efficiently isolates immune cells from prostate tissue, enabling detailed analysis for benign prostatic hyperplasia and prostate cancer research. This technique improves cell viability and supports advancements in immunotherapy.

Keywords:
cell isolationimmune cell populationsmulticolor flow cytometryprostate tissuesingle cell suspension

More Related Videos

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
11:56

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells

Published on: May 15, 2014

8.4K
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

12.7K

Related Experiment Videos

Last Updated: Apr 26, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
07:16

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

13.8K
In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
11:56

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells

Published on: May 15, 2014

8.4K
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

12.7K

Area of Science:

  • Urology
  • Immunology
  • Oncology

Background:

  • Benign prostatic hyperplasia (BPH) and prostate cancer are prevalent in men over 50.
  • Immune cells in prostate lesions may influence tumor progression.
  • Isolating prostate immune cells for study is challenging due to low numbers and material access.

Purpose of the Study:

  • To develop an efficient method for isolating immune cells from prostate tissue.
  • To enable phenotypic characterization of these immune cells for research.

Main Methods:

  • A novel protocol was established to process fresh prostate tissue from BPH patients.
  • Immune cells were isolated for multicolor flow cytometry analysis.
  • The method avoids enzymatic digestion and 37°C incubation to preserve cell integrity.

Main Results:

  • The method successfully isolated various immune cell populations from all patient samples.
  • Median cellular viability was high at 90%.
  • Significant variation in immune cell numbers and frequencies (e.g., CD3- 87%, CD3+ 15%) was observed between patients.

Conclusions:

  • This method facilitates phenotypic characterization of immune cells in prostate tissue.
  • It supports research into the role of immune cells in prostate cancer.
  • The technique may advance the development of novel immunotherapies for prostate cancer.