Related Experiment Video
Updated: Apr 26, 2026
09:34
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
Published on: February 6, 2020
7.2K
Sortase-based bio-organic strategies for macromolecular synthesis
V Haridas1, Sandhya Sadanandan, N U Dheepthi
1Department of Chemistry, Indian Institute of Technology Delhi, New Delhi 110016 (India). haridasv@iitd.ac.in.
Chembiochem : a European Journal of Chemical Biology
|August 12, 2014
Summary
Sortase A (SrtA) enables precise protein ligation, a crucial reaction for complex molecules. This chemoselective method holds significant promise for future applications in chemistry, biology, and medicine.
Area of Science:
- Biochemistry
- Chemical Biology
Background:
- Sortase A (SrtA) is a naturally occurring enzyme facilitating protein ligation.
- Chemoselective reactions are vital for manipulating complex biomolecules.
Purpose of the Study:
- To highlight the capabilities of Sortase A in protein ligation.
- To underscore the potential of Sortase-mediated ligation (SML) in various scientific fields.
Main Methods:
- Utilizing Sortase A for protein ligation.
- Employing chemoselective reactions for molecular assembly.
Main Results:
- Demonstrated the efficiency of SrtA in performing ligation on complex protein molecules.
- Established SML as a powerful tool for precise molecular construction.
Conclusions:
- Sortase-mediated ligation (SML) is a versatile and effective technique.
- SML is poised for broad applications in chemistry, biology, and medicine due to its chemoselectivity.
Related Concept Videos
01:28ATP and Macromolecule Synthesis
5.1K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
5.1K
02:55Synthetic Biology
4.4K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Golden rice
Golden rice is a genetically modified...
4.4K
01:24Biosynthesis in Bacteria
1.0K
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
1.0K