Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unlocking Wafer-Scale 3D Photonic Systems With Ion-Beam-Induced Origami.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Unveiling and steering Thiobacillus denitrificans metabolic network to tackle denitrification inhibition under antibiotic-heavy metal co-exposure.

Bioresource technology·2026
Same author

Antibiotics regulate bacterial diversification, niche expansion and evolution under primordial conditions.

Biochimie·2026
Same author

How the Electrochemical Double Layer Manipulates Molecule-Metal Interactions.

ACS nano·2026
Same author

Optical convolutional spectrometer.

Nature photonics·2026
Same author

Machine learning methods identified cellular senescence-related hub molecules in sepsis-induced acute respiratory distress syndrome (ARDS) and their upstream regulatory network.

Molecular biology reports·2026

Related Experiment Video

Updated: Apr 26, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.2K

Watching individual molecules flex within lipid membranes using SERS.

Richard W Taylor1, Felix Benz1, Daniel O Sigle2

  • 11] NanoPhotonics Centre, Cavendish Laboratory, Department of Physics, JJ Thompson Ave, University of Cambridge, Cambridge, CB3 0HE, UK [2].

Scientific Reports
|August 13, 2014
PubMed
Summary

This study uses Raman spectroscopy with gold nanoparticles to track individual lipid molecules in cell membranes, revealing their dynamic structural and conformational changes in real-time.

More Related Videos

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

3.9K
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.2K

Related Experiment Videos

Last Updated: Apr 26, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.2K
Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

3.9K
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.2K

Area of Science:

  • Biophysics
  • Spectroscopy
  • Nanotechnology

Background:

  • Understanding biomolecular dynamics within cell membranes is crucial for deciphering life processes.
  • Raman spectroscopy offers non-destructive molecular fingerprinting for dynamic analysis.
  • Current methods often lack the resolution to track individual molecules in situ.

Purpose of the Study:

  • To develop and demonstrate a technique for high-speed, single-molecule investigation of lipid dynamics in bio-membranes.
  • To connect molecular structure and function by tracking molecular movements and conformational changes.
  • To enhance Raman spectroscopy's capabilities for probing nanoscale environments.

Main Methods:

  • Utilized a 'nanoparticle-on-mirror' geometry with gold nano-components to create intense localized optical fields.
  • Employed Raman spectroscopy to analyze molecular vibrations and identify diffusing lipids.
  • Achieved a probed volume of a few nm³ for high-resolution, repeated measurements on single molecules.

Main Results:

  • Successfully observed and measured individual lipids undergoing conformational changes within model bio-membranes.
  • Detected molecular flexing through characteristic rapid, correlated vibrational shifts and intensity fluctuations in Raman spectra.
  • Demonstrated the ability to track molecular interactions and environmental influences on lipid dynamics.

Conclusions:

  • The developed technique enables in situ, high-speed single-molecule investigations of membrane components.
  • This method provides unprecedented insights into the hidden dynamics of cell membranes.
  • Offers a novel approach to study biomolecular behavior critical for various life processes.