Related Experiment Video
Updated: Apr 25, 2026
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
Trends in antifungal susceptibility of Candida species--one year observation
Abstract:
In the past years opportunistic fungal infections have seriously increased, mainly in immunocompromised patients. The aim of the study was to determine the prevalence of yeast-like fungi in invasive candidiasis and to estimate its susceptibility to chosen antifungal agents. One hundred and sixty strains of yeast-like fungi were cultured from various clinical material: samples from lower respiratory tract, blood, the peritoneal cavity and others. The susceptibility tests were established according to the quantitative E-test method. The Candida genus represented the main etiological factor of invasive candidiasis. The predominant species were: C. glabrata (71/160), C. albicans (34/160), C. krusei (17/160), C. tropicalis (14/160). All tested strains were the most resistant to itraconazole. Candida glabrata presented the 100% susceptibility to amphotericin B and caspofungin and was the least susceptible to itraconazole, posaconazole and voriconazole. Candida albicans was the most susceptible species to all antymicotics.
Insights
Opportunistic fungal infections are rising in immunocompromised patients. This study found Candida glabrata and Candida albicans were common causes of invasive candidiasis, with varying antifungal susceptibility.
Area of Science:
- Medical Mycology
- Infectious Diseases
- Clinical Microbiology
Background:
- Opportunistic fungal infections, particularly invasive candidiasis, have increased, especially in immunocompromised individuals.
- The Candida genus is a significant cause of invasive fungal infections worldwide.
- Understanding the prevalence and antifungal susceptibility patterns of yeast-like fungi is crucial for effective treatment.
Purpose of the Study:
- To determine the prevalence of yeast-like fungi causing invasive candidiasis.
- To assess the susceptibility of these fungal isolates to key antifungal agents.
Main Methods:
- Culturing of 160 yeast-like fungal strains from diverse clinical samples (respiratory, blood, peritoneal fluid, etc.).
- Quantitative E-test method employed for antifungal susceptibility testing.
- Identification of predominant Candida species: C. glabrata, C. albicans, C. krusei, and C. tropicalis.
Main Results:
- Candida genus was the primary etiological agent of invasive candidiasis.
- Candida glabrata (71/160) and Candida albicans (34/160) were the most prevalent species.
- All strains showed the highest resistance to itraconazole. C. glabrata exhibited 100% susceptibility to amphotericin B and caspofungin but was least susceptible to itraconazole, posaconazole, and voriconazole. C. albicans was most susceptible to all tested antifungals.
Conclusions:
- Candida species, particularly C. glabrata and C. albicans, are major contributors to invasive candidiasis.
- Antifungal susceptibility varies significantly among Candida species, with notable resistance to itraconazole.
- C. glabrata demonstrates high susceptibility to amphotericin B and caspofungin, while C. albicans remains broadly susceptible to tested agents, guiding therapeutic strategies.
More Related Videos
08:54Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
07:19A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
Published on: October 21, 2010
