[Clonal propagation of Gynostemma pentaphyllum (Thumb.) Makino in test tubes]

The explants taken from adventitious shoots or nodes of adventitious bud of Gynostemma pentaphyllum produced numerous shoots on MS medium supplemented with BA 1mg/L and IAA 0.05 mg/L. When the segments of shoots were grown on the 1/2MS medium supplemented with IBA 1 mg/L, roots were initiated differentiating to plantlets. Substitution of polyurethane sponge for agar can accelerate the growth of platlets.