An electrochemical immunosensor based on interdigitated array microelectrode for the detection of chlorpyrifos

Area of Science:

• Electrochemistry
• Biosensors
• Analytical Chemistry

Background:

• Pesticide detection is crucial for environmental and food safety.
• Existing methods for chlorpyrifos analysis can be time-consuming or expensive.
• Development of rapid, sensitive, and cost-effective detection tools is needed.

Purpose of the Study:

• To develop a novel electrochemical immunosensor for sensitive and specific detection of chlorpyrifos.
• To utilize interdigitated array microelectrodes (IDAMs) for enhanced signal transduction.
• To establish a rapid screening method for chlorpyrifos in diverse sample matrices.

Main Methods:

• Fabrication of an immunosensor by immobilizing anti-chlorpyrifos antibodies onto gold IDAMs via protein A.
• Detection of chlorpyrifos based on impedance changes measured by electrochemical impedance spectroscopy.
• Optimization of sensor parameters for maximal sensitivity and specificity.

Main Results:

• The immunosensor demonstrated a linear response to chlorpyrifos concentrations from 10(0) to 10(5) ng/mL.
• A low detection limit of 0.014 ng/mL for chlorpyrifos was achieved.
• The sensor exhibited high sensitivity, specificity, and rapidity.

Conclusions:

• The developed electrochemical immunosensor provides a sensitive, specific, and rapid method for chlorpyrifos detection.
• The low cost and ease of use make it suitable for screening pesticide residues in environmental, agricultural, and pharmaceutical samples.
• This technology offers a valuable tool for routine pesticide monitoring and safety assessment.