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Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Updated: Apr 25, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Proteomic revelations.

Jeffrey M Davidson1

  • 1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA; Research Service, VA Tennessee Valley Healthcare System, Nashville, Tennessee, USA.

The Journal of Investigative Dermatology
|August 15, 2014
PubMed
Summary
This summary is machine-generated.

Proteomics reveals common cellular issues in systemic sclerosis and epidermolysis bullosa. Fibroblasts show senescence and autophagy deficiencies, increasing cellular stress and disease severity.

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Area of Science:

  • Cellular biology
  • Proteomics
  • Dermatology

Background:

  • Systemic sclerosis and recessive dystrophic epidermolysis bullosa are debilitating skin disorders.
  • Fibroblasts play a critical role in skin structure and repair.
  • Both conditions place significant metabolic demands on skin fibroblasts.

Purpose of the Study:

  • To investigate the proteomic profiles of skin fibroblasts in systemic sclerosis and recessive dystrophic epidermolysis bullosa.
  • To identify common molecular mechanisms underlying these distinct fibrotic and blistering disorders.

Main Methods:

  • Proteomic analysis of cultured skin fibroblasts.
  • Comparison of protein expression patterns between patient groups and controls.

Main Results:

  • Common findings of cellular senescence in fibroblasts from both disease groups.
  • Identified deficiencies in autophagy, a key cellular degradation pathway.
  • Senescence and impaired autophagy contribute to increased cellular stress.

Conclusions:

  • Senescence and autophagy deficiency are convergent mechanisms in systemic sclerosis and recessive dystrophic epidermolysis bullosa.
  • Targeting senescence or enhancing autophagy may offer therapeutic strategies for these conditions.