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Updated: Apr 25, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Published on: September 30, 2016
The "tale" of poly(A) binding protein: the MLLE domain and PAM2-containing proteins
Jingwei Xie1, Guennadi Kozlov1, Kalle Gehring1
1Department of Biochemistry and the Groupe de recherche axé sur la structure des protéines, McGill University, 3649 Promenade Sir-William-Osler, Montréal, Québec H3G 0B1, Canada.
Abstract:
The cytoplasmic poly(A) binding protein 1 (PABPC1) is an essential eukaryotic translational initiation factor first described over 40 years ago. Most studies of PABPC1 have focused on its N-terminal RRM domains, which bind the mRNA 3' poly(A) tail and 5' translation complex eIF4F via eIF4G; however, the protein also contains a C-terminal MLLE domain that binds a peptide motif, termed PAM2, found in many proteins involved in translation regulation and mRNA metabolism. Studies over the past decade have revealed additional functions of PAM2-containing proteins (PACs) in neurodegenerative diseases, circadian rhythms, innate defense, and ubiquitin-mediated protein degradation. Here, we summarize functional and structural studies of the MLLE/PAM2 interaction and discuss the diverse roles of PACs.
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