Alpha, mu and pi glutathione S-transferases: species (Talpa europaea) differences in their expression

1. Tissue cytosols from Talpa europaea were examined for their glutathione S-transferase isoenzyme content by chromatofocusing, inhibition and immunological techniques and the results compared with data from adult human tissue cytosols. 2. Two sets of glutathione S-transferase isoenzymes were found in liver cytosol of Talpa europaea, they demonstrated similar properties to human alpha and mu isoforms. 3. There was no evidence of expression of the pi isoenzyme set in any of the tissues studied and in this respect Talpa europaea differs from other mammalian species studied so far.