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Updated: Apr 25, 2026

Methodology for Accurate Detection of Mitochondrial DNA Methylation
Published on: May 20, 2018
The comparison of two complete mitochondrial genomes of Pseudobagrus ondon (Siluriformes, Bagridae)
Haipeng Zhao1,2, Donglin Yuan3, Wen Luo2
1a School of Life Science, Henan University , Kaifeng , P. R. China .
Abstract:
The complete mitochondrial genome of Pseudobagrus ondon collected from the Qiantang River in Zhejiang Province was determined in this study with polymerase chain reaction. It contained 22 tRNA genes, 13 protein-coding genes, 2 rRNAs and a control region with the base composition 31.03% A, 27.92% C, 25.78% T and 15.27% G. It shared the arrangement pattern that was identical with most vertebrates. Here we compared the complete mitochondrial genome with another Pseudobagrus ondon species has been sequenced before discovering some differences between the two entities belong to the same species. The complete genome of the one in this study was 16,531 bp in length but the other was 16,534 bp. The variable sites and the genetic distances between them were 105 bp and 0.7%, respectively. There was 79 variable sites occurred in the protein-coding genes except that gene ATP8 and ND3 were the same sequences in the two mitogenomes. Considering that relatively little work has been done on the fishes of Pseudobagrus, we hope this study could make some contribution for the phylogenetic relationships of this group of fishes.
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