Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Electrical Double Layer01:30

The Electrical Double Layer

223
In the region where two bulk phases meet, an intricate electric charge distribution arises due to charge transfer, ion adsorption, molecular orientation, and charge distortion. This complex distribution is commonly referred to as the electrical double layer.When a solid electrode interfaces with ions in an electrolyte solution, the speed of electron transfer dictates the rates of oxidation and reduction. The electrode acquires a charge through the escape of atoms into the solution as cations or...
223
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.1K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.1K
Processes at Electrodes01:30

Processes at Electrodes

95
The electrode interacts with ions in the electrolyte solution at its interface. The rate of oxidation and reduction depends on the speed at which electrons can transfer through this interface. As ions attach to or leave the electrode surface, the electrode acquires a charge, and an electrical potential forms across the interface, making the process more difficult to reach equilibrium. The charge on the electrode affects the local ion concentrations in the solution, though thermal motion...
95
Electrostatic Boundary Conditions in Dielectrics01:27

Electrostatic Boundary Conditions in Dielectrics

2.1K
When an electric field passes from one homogeneous medium to another, crossing the boundary between the two mediums imparts a discontinuity in the electric field. This results in electrostatic boundary conditions that depend on the type of mediums the field propagates through.
Consider a case where both the mediums across a boundary are two different dielectric materials. Recall that the electric field and electric displacement are proportional and related through the material's permittivity....
2.1K
Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

987
Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current...
987
Electric Field at the Surface of a Conductor01:26

Electric Field at the Surface of a Conductor

4.5K
Consider a conductor in electrostatic equilibrium. The net electric field inside a conductor vanishes, and extra charges on the conductor reside on its outer surface, regardless of where they originate.
In the 19th century, Michael Faraday conducted the famous ice pail experiment to prove that the charges always reside on the surface of a conductor. The experimental set-up consists of a conducting uncharged container mounted on an insulating stand. The outer surface of the container is...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cooperativity in Enzyme-Substrate Complex Formation in Nitrogenase-like Dark Operative Protochlorophyllide Oxidoreductase (DPOR).

Chimia·2026
Same author

Covalent Attachment of Molecularly Thin PVC Membrane by Click Chemistry for Ionophore-Based Ion Sensors.

Analytical chemistry·2025
Same author

Enzyme-Substrate Complex Formation and Electron Transfer in Nitrogenase-Like Dark-Operative Protochlorophyllide Oxidoreductase (DPOR).

ChemistryOpen·2025
Same author

Mesoporous Electrodes Enhance the Electrocatalytic Performance of [FeFe]-Hydrogenase.

Angewandte Chemie (International ed. in English)·2024
Same author

Understanding protein-nanoparticle interactions leading to protein corona formation: In vitro - in vivo correlation study.

International journal of biological macromolecules·2023
Same author

Facile Functionalization of Carbon Electrodes for Efficient Electroenzymatic Hydrogen Production.

JACS Au·2023

Related Experiment Video

Updated: Apr 25, 2026

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy
10:49

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy

Published on: November 28, 2014

21.4K

Electric double-layer potentials and surface regulation properties measured by colloidal-probe atomic force

F Javier Montes Ruiz-Cabello1, Gregor Trefalt1, Plinio Maroni1

  • 1Department of Inorganic and Analytical Chemistry, University of Geneva, Sciences II, Quai Ernest-Ansermet 30, 1205 Geneva, Switzerland.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|August 15, 2014
PubMed
Summary

The colloidal-probe technique accurately measures water-solid interface charging parameters, including diffuse-layer potential and charge regulation. This atomic force microscope method is versatile for various surfaces and salt conditions, though limited by salt concentration and particle size.

More Related Videos

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM
08:31

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM

Published on: February 10, 2021

6.4K
Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
13:15

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy

Published on: July 18, 2014

10.2K

Related Experiment Videos

Last Updated: Apr 25, 2026

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy
10:49

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy

Published on: November 28, 2014

21.4K
Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM
08:31

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM

Published on: February 10, 2021

6.4K
Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
13:15

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy

Published on: July 18, 2014

10.2K

Area of Science:

  • Surface science
  • Colloid and interface science
  • Physical chemistry

Background:

  • Accurate characterization of water-solid interfaces is crucial for understanding phenomena like adhesion, wetting, and colloidal stability.
  • Existing methods for determining interface charging parameters often lack precision or versatility.
  • Understanding diffuse-layer potential and charge regulation is essential for predicting inter-surface forces.

Purpose of the Study:

  • To demonstrate the utility of the colloidal-probe technique for precisely quantifying water-solid interface charging parameters.
  • To establish a method for determining both diffuse-layer potential and charge regulation properties.
  • To highlight the technique's applicability to diverse substrates and ionic environments.

Main Methods:

  • Utilizing atomic force microscopy (AFM) with a colloidal probe to measure forces between interfaces.
  • Employing Poisson-Boltzmann (PB) theory to interpret force profiles and extract charging parameters.
  • Calibrating a highly charged probe particle in a symmetric sphere-sphere geometry.
  • Measuring force profiles in asymmetric sphere-sphere or sphere-plane geometries to characterize unknown substrates.

Main Results:

  • The colloidal-probe technique accurately determines diffuse-layer potential and charge regulation parameters.
  • The method successfully quantifies surface charge properties for various substrates.
  • The technique is effective in salt solutions, including those with multivalent ions.

Conclusions:

  • The colloidal-probe technique offers a versatile and accurate approach to characterizing water-solid interface electrical properties.
  • The ability to quantify charge regulation provides critical insights into inter-surface interactions.
  • Future work may extend the technique's applicability to higher salt concentrations and smaller particle sizes.