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Related Experiment Videos

Leydig cell and extracellular matrix effects on Sertoli cell function: biochemical and morphologic studies.

J Reventos1, M H Perrard-Sapori, P G Chatelain

  • 1INSERM U 307, Hopital Debrousse, Lyon, France.

Journal of Andrology
|September 1, 1989
PubMed
Summary

Leydig and Sertoli cells from pig testes show reciprocal influence, enhancing steroidogenesis and hormone receptor expression. Extracellular matrix significantly boosts these positive effects and cell organization.

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Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Endocrinology

Background:

  • Leydig and Sertoli cells are crucial for testicular function.
  • Understanding their reciprocal interactions is key to male reproductive health.
  • Previous studies suggest hormonal influences but lack detailed coculture insights.

Purpose of the Study:

  • To investigate the reciprocal influence between pig Leydig and Sertoli cells.
  • To determine the role of extracellular matrix in modulating cell interactions.
  • To examine the effects of follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG) on cell function.

Main Methods:

  • Coculture of pig Leydig and Sertoli cells in standard plastic dishes versus dishes coated with basement membrane matrix.

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  • Treatment with pFSH and hCG to assess steroidogenic response and receptor expression.
  • Measurement of FSH binding sites and plasminogen activator activity.
  • Microscopic analysis of cell organization under different culture conditions.

    • Main Results:

    • Sertoli cells enhanced Leydig cell steroidogenic response to hCG.
    • PFSH pretreatment increased Leydig cell steroidogenic capacity and hCG receptors.
    • Coculture with Leydig cells increased Sertoli cell FSH binding sites and FSH-induced plasminogen activator activity.
    • Extracellular matrix significantly enhanced these reciprocal tropic effects and promoted cell clustering (Leydig cells external, Sertoli cells internal).

    Conclusions:

    • Pig Leydig and Sertoli cells exhibit significant positive reciprocal interactions.
    • Extracellular matrix plays a critical role in enhancing these interactions and promoting organized cell structures.
    • These findings provide insights into the complex microenvironment regulating testicular function.