Hyaluronan in aged collagen matrix increases prostate epithelial cell proliferation
Mamatha Damodarasamy1, Robert B Vernon, Christina K Chan
1Department of Medicine, University of Washington, Harborview Medical Center, 325 9th Avenue, Box 359625, Seattle, WA, 98104, USA.
In Vitro Cellular & Developmental Biology. Animal
|August 16, 2014
Summary
Aging prostate extracellular matrix (ECM) changes, specifically increased hyaluronan (HA), promote benign prostatic hyperplasia (BPH) and cancer cell growth. Aged collagen gels accelerated the proliferation of prostate cells, indicating HA
Area of Science:
- Biochemistry
- Cell Biology
- Oncology
Background:
- The prostate's extracellular matrix (ECM), mainly collagen, disorganizes with age, potentially driving hyperplasia and cancer.
- Collagen extracts from aged mice mimic aged tissue collagen, useful for studying cell-ECM interactions in 3D gels.
Purpose of the Study:
- To investigate how aged versus young mouse collagen ECM affects human prostate cell line behavior.
- To identify specific age-associated ECM components influencing prostate cell proliferation.
Main Methods:
- Cultured normal prostate epithelial cells (PEC), BPH-1, and LNCaP cells on 3D gels of young and aged mouse collagen extracts.
- Analyzed ECM composition, focusing on collagen and glycosaminoglycans like hyaluronan (HA).
- Assessed the impact of exogenous HA on cell proliferation in young collagen gels.
Main Results:
- Prostate cell proliferation (PEC, BPH-1, LNCaP) increased on aged collagen gels compared to young ones.
- Aged collagen gels showed significantly higher hyaluronan (HA) content than young gels.
- Adding HA to young collagen gels increased BPH-1 cell proliferation but not PEC or LNCaP cells.
- Human prostate tissues revealed higher stromal HA expression in BPH and cancer compared to normal tissue.
Conclusions:
- Age-related increases in prostate ECM hyaluronan (HA) contribute to prostatic epithelial growth.
- Altered ECM composition, particularly elevated HA, is implicated in the pathogenesis of benign prostatic hyperplasia and prostate cancer.
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