Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic α-1-microglobulin ameliorates kidney ischemia-reperfusion injury.

American journal of physiology. Renal physiology·2024
Same author

Proximal tubule role in albumin homeostasis: controversy, species differences, and the contributions of intravital microscopy.

Kidney international·2023
Same author

The Effects of Peroxisome Proliferator-Activated Receptor-Delta Modulator ASP1128 in Patients at Risk for Acute Kidney Injury Following Cardiac Surgery.

Kidney international reports·2023
Same author

Is Albumin Toxic to the Kidney?: It Depends.

Clinical journal of the American Society of Nephrology : CJASN·2023
Same author

Lrpap1 (RAP) Inhibits Proximal Tubule Clathrin Mediated and Clathrin Independent Endocytosis, Ameliorating Renal Aminoglycoside Nephrotoxicity.

Kidney360·2023
Same author

Defining the Intravital Renal Disposition of Fluorescence-Quenched Exenatide.

Molecular pharmaceutics·2023

Related Experiment Video

Updated: Apr 25, 2026

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
11:13

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

Published on: April 17, 2013

10.0K

Using 2-photon microscopy to understand albuminuria.

Bruce A Molitoris1

  • 1Indianapolis, Indiana.

Transactions of the American Clinical and Climatological Association
|August 16, 2014
PubMed
Summary

Intravital two-photon microscopy allows real-time, four-dimensional visualization of cellular processes. This advanced imaging challenges kidney filtration paradigms and clarifies the role of proximal tubules in albuminuria.

Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Renal Physiology

Background:

  • Intravital microscopy and fluorescent probes have revolutionized the study of cellular processes.
  • Four-dimensional (4D) imaging with subcellular resolution enables real-time analysis of dynamic biological events.
  • Advanced techniques allow simultaneous tracking of multiple molecules within living tissues.

Purpose of the Study:

  • To highlight the capabilities of intravital two-photon microscopy for studying intra-vital cell biology.
  • To investigate the physiological filtration of albumin in rat kidneys.
  • To elucidate the role of proximal tubules in albuminuria.

Main Methods:

  • Utilizing intravital two-photon microscopy with fluorescent probes and advanced software.

More Related Videos

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
11:47

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

2.1K
Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
07:37

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

Published on: October 11, 2018

11.8K

Related Experiment Videos

Last Updated: Apr 25, 2026

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
11:13

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

Published on: April 17, 2013

10.0K
Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
11:47

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

2.1K
Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
07:37

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

Published on: October 11, 2018

11.8K
  • Employing transgenic fluorescently labeled proteins, plasmid delivery, and photo-activatable probes.
  • Performing quantitative analysis and direct visualization of rat glomeruli and proximal tubules.

    • Main Results:

    • Demonstrated the capacity to quantify distribution, behavior, and interactions of multiple probes in 4D.
    • Provided direct visualization and quantitative analysis of albumin handling in rat kidneys.
    • Challenged the established paradigm of limited albumin filtration under physiological conditions.

    Conclusions:

    • Intravital two-photon microscopy significantly enhances the understanding of intra-vital cellular and subcellular biology.
    • The study questions traditional views on albumin filtration and highlights the critical role of proximal tubules in albuminuria.
    • Direct visualization provides new insights into renal physiology and disease mechanisms.