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Gender determination from pulpal tissue.

Manisha M Khorate1, Anita Dhupar2, Junaid Ahmed3

  • 1Department of Oral Medicine, Diagnosis, and Radiology, Goa Dental College and Hospital, Bambolim, Goa, India.

Journal of Forensic Dental Sciences
|August 16, 2014
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Summary

Gender determination using dental pulp is reliable up to 7 weeks. Analyzing Barr bodies (BB) and F bodies (FB) in pulp tissue offers a cost-effective forensic method.

Keywords:
Barr bodiesF-bodiesforensic odontologygender determinationpulpal tissue

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Area of Science:

  • Forensic Odontology
  • Genetics
  • Cell Biology

Background:

  • Dental pulp tissue contains cellular material suitable for genetic analysis.
  • Sex chromosome analysis (Barr body and F body) is a standard method for gender determination.

Purpose of the Study:

  • To assess the efficacy of Barr body (BB) and F body (FB) detection in dental pulp for gender identification.
  • To determine the reliability of this method over time post-extraction.

Main Methods:

  • 100 extracted teeth (50 male, 50 female) were analyzed.
  • Dental pulp tissue was stained for Barr bodies (BB) using Hemotoxylin and Eosin and F bodies (FB) using Quinacrine dihydrochloride.
  • Microscopic examination was performed on samples collected up to 49 days post-extraction.

Main Results:

  • Gender determination from dental pulp was feasible up to 7 weeks post-extraction.
  • The detection rates of Barr bodies (BB) and F bodies (FB) decreased over time.
  • A predictive equation was developed using discriminant function analysis.

Conclusions:

  • Detecting Barr bodies (BB) and F bodies (FB) in dental pulp is a reliable and cost-effective method for gender determination.
  • This technique holds significant potential for forensic applications.