Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

65.0K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
65.0K
Membrane Proteins01:30

Membrane Proteins

18.5K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
18.5K
Membrane Proteins01:30

Membrane Proteins

4.3K
4.3K
Membrane Fluidity01:26

Membrane Fluidity

13.9K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
13.9K
Membrane Fluidity01:23

Membrane Fluidity

150.0K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
150.0K
Fluid Mosaic Model01:19

Fluid Mosaic Model

14.5K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
14.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complementary Front Doors, Shared Back Door: Integrating Blood-Based CRC Screening Without Overwhelming Colonoscopy-By Far North and Online Colorectal Alliance (FNOCA).

ANZ journal of surgery·2026
Same author

Declining Medicare Work Relative Value Unit Reimbursement in Orthopaedic Surgery: A Cross-Specialty Analysis from 2000 to 2025, with Projections to 2030.

JB & JS open access·2026
Same author

From extracellular entry to intracellular release: A water-assisted transport cycle for creatine in SLC6A8.

Protein science : a publication of the Protein Society·2026
Same author

Rare coding variant architecture and gene discovery from 130,000 sequenced cases of atrial fibrillation.

Research square·2026
Same author

Strategic consensus on the clinical translation of advanced therapies in paediatric rare neurological disorders.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2026
Same author

Modelling synaptic dysfunction in childhood dementia using human iPSC-derived cortical networks.

Nature communications·2026

Related Experiment Video

Updated: Apr 25, 2026

Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP
08:14

Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP

Published on: April 20, 2015

17.3K

ProBLM web server: protein and membrane placement and orientation package.

Taylor Kimmett1, Nicholas Smith2, Shawn Witham2

  • 1Department of Computer Science, Clemson University, Clemson, SC 29634, USA ; Computational Biophysics and Bioinformatics, Department of Physics, Clemson University, Clemson, SC 29634, USA.

Computational and Mathematical Methods in Medicine
|August 16, 2014
PubMed
Summary

Researchers developed a geometry-based method to insert membrane proteins into lipid bilayers. This ProBLM web server models protein-membrane interactions, aiding studies on membrane protein function.

More Related Videos

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

12.8K
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

5.3K

Related Experiment Videos

Last Updated: Apr 25, 2026

Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP
08:14

Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP

Published on: April 20, 2015

17.3K
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

12.8K
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

5.3K

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Membrane protein structures are often determined outside their native lipid bilayer environment.
  • Understanding the influence of the membrane on protein characteristics requires accurate positioning within a bilayer.

Purpose of the Study:

  • To develop a computational method for inserting known 3D membrane protein structures into lipid bilayers.
  • To provide a tool for studying the role of membrane environment on protein structure and function.

Main Methods:

  • A geometry-based approach was used to automatically insert proteins into pre-generated lipid bilayers or pseudomembranes.
  • A pseudomembrane was constructed using the Protein Nano-Object Integrator for desolvation effect modeling.
  • The ProBLM web server was implemented, allowing users to upload protein structures and regenerate missing atoms.

Main Results:

  • The ProBLM server successfully integrates membrane proteins into various lipid bilayer models and pseudomembranes.
  • The tool allows for manual refinement of protein position and orientation within the membrane.
  • It facilitates the creation of realistic protein-membrane complexes for further analysis.

Conclusions:

  • The ProBLM server offers a valuable, automated solution for modeling membrane proteins within their native-like environments.
  • This approach aids in understanding membrane protein behavior and function by considering lipid interactions.
  • The freely available web server supports biophysical research by simplifying the creation of protein-membrane complexes.