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Gene expression signatures affected by ethanol and/or nicotine in normal human normal oral keratinocytes (NHOKs)
Jeffrey J Kim1, Omar Khalid1, Lewei Duan2
1Laboratory of Stem Cell and Cancer Epigenetic Research, UCLA School of Dentistry, Los Angeles, CA, USA.
Abstract:
It has been reported that nicotine/alcohol alter epigenetic control and lead to abrogated DNA methylation and histone modifications, which could subsequently perturb transcriptional regulation critically important in cellular transformation. The aim of this study is to determine the molecular mechanisms of nicotine/alcohol-induced epigenetic alterations and their mechanistic roles in transcriptional regulation in human adult stem cells. We hypothesized that nicotine/alcohol induces deregulation of epigenetic machinery and leads to epigenetic alterations, which subsequently affect transcriptional regulation in oral epithelial stem cells. As an initiating step we have profiled transcriptomic alterations induced by combinatory administration of EtOH and nicotine in primary normal human oral keratinocytes. Here we provide detailed experimental methods, analysis and information associated with our data deposited into Gene Expression Omnibus (GEO) under GSE57634. Our data provide comprehensive transcriptomic map describing molecular changes induced by EtOH and nicotine on normal human oral keratinocytes.
Insights
Nicotine and alcohol exposure disrupt epigenetic control in oral stem cells, altering DNA methylation and gene expression. This study maps these transcriptomic changes to understand cellular transformation mechanisms.
Area of Science:
- Epigenetics
- Molecular Biology
- Stem Cell Biology
Background:
- Nicotine and alcohol are known to affect epigenetic regulation.
- Epigenetic alterations, including DNA methylation and histone modifications, can disrupt gene expression.
- These disruptions are critical in cellular transformation processes.
Purpose of the Study:
- To investigate the molecular mechanisms underlying nicotine/alcohol-induced epigenetic changes.
- To determine the role of these epigenetic alterations in transcriptional regulation.
- To examine these effects in human adult stem cells, specifically oral epithelial stem cells.
Main Methods:
- Profiling transcriptomic alterations.
- Combinatory administration of ethanol (EtOH) and nicotine.
- Utilizing primary normal human oral keratinocytes.
- Data deposited in Gene Expression Omnibus (GEO) under GSE57634.
Main Results:
- Comprehensive transcriptomic map generated.
- Detailed molecular changes induced by EtOH and nicotine identified.
- Provided experimental methods, analysis, and associated data.
Conclusions:
- Nicotine and alcohol induce significant transcriptomic alterations in oral keratinocytes.
- The study provides a foundation for understanding the epigenetic basis of cellular changes induced by these substances.
- The data serves as a resource for further research into oral carcinogenesis and stem cell deregulation.
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