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Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
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Related Experiment Video

Updated: Apr 25, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

A timeless but timely connection between replication and recombination.

Valérie Borde¹, Michael Lichten²

¹CNRS UMR3664, Institut Curie, Centre de Recherche, Paris 75248, France.

|August 16, 2014

Summary

This summary is machine-generated.

DNA double-strand break formation, crucial for meiotic recombination, is coordinated with DNA replication. This coordination depends on the TIM-TIPIN complex recruiting a key kinase to the replication fork.

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Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Meiotic recombination initiates with DNA double-strand breaks (DSBs).
The timing of DSB formation is coordinated with DNA replication during meiosis.
Understanding this coordination is key to meiosis and genome stability.

Purpose of the Study:

To elucidate the molecular mechanism coordinating DSB formation with replication.
To identify the proteins involved in recruiting key enzymes to the replication fork.

Main Methods:

Utilized genetic and biochemical approaches in yeast models.
Investigated the role of the TIM-TIPIN complex in recruiting Dbf4-dependent kinase (DDK).
Analyzed DSB formation and replication fork progression.

Main Results:

Demonstrated that the conserved TIM-TIPIN complex recruits Dbf4-dependent kinase (DDK) to the replication fork.
Showed that this recruitment is essential for the temporal coordination of DSB formation with replication.
Identified a conserved mechanism linking replication and recombination initiation.

Conclusions:

The TIM-TIPIN complex acts as a scaffold, linking replication machinery to DSB formation machinery.
This mechanism ensures DSBs occur at the appropriate time during S-phase.
The findings suggest this regulatory pathway may also control other replication-associated processes.