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Screen-printed microfluidic dielectrophoresis chip for cell separation.

Hongwu Zhu1, Xiaoguang Lin2, Yong Su2

  • 1Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China; School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, China.

Biosensors & Bioelectronics
|August 16, 2014
PubMed
Summary

We developed an inexpensive screen-printed microfluidic chip for dielectrophoresis (DEP) applications. This novel fabrication method enables high-throughput mass production of DEP devices for efficient bioparticle manipulation.

Keywords:
Cell separationDielectrophoresisMicrofluidics chipScreen-printing

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Area of Science:

  • Microfluidics
  • Biophysics
  • Materials Science

Background:

  • Dielectrophoresis (DEP) is effective for manipulating micro/nano-scale bioparticles in microfluidic systems.
  • Conventional fabrication methods for DEP devices are complex, costly, and limit throughput.
  • There is a need for simplified, cost-effective fabrication of DEP microfluidic chips.

Purpose of the Study:

  • To report a novel, inexpensive microfluidic alternating current DEP (AC-DEP) chip.
  • To demonstrate a fabrication method suitable for high-throughput mass production.
  • To evaluate the performance of the fabricated AC-DEP chip for bioparticle separation.

Main Methods:

  • Fabrication of AC-DEP chip using a layer-by-layer screen printing process.
  • Utilizing carbon paste to print interdigitated electrodes with a semi-3D structure.
  • Employing yeast cells and PS microspheres as model particles for separation experiments.

Main Results:

  • Achieved extreme simplicity in chip fabrication through screen printing.
  • Reduced chip cost significantly by using carbon paste instead of metals.
  • Demonstrated high capture rate and separation efficiency for yeast cells from microspheres.

Conclusions:

  • Screen printing offers a cost-effective and high-throughput method for AC-DEP chip fabrication.
  • The novel carbon electrode design enhances particle trapping efficiency.
  • The developed AC-DEP chip is suitable for efficient bioparticle separation and characterization.