Related Experiment Video
Updated: Apr 25, 2026

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
Published on: February 27, 2015
Effect of surface stress on microcantilever resonance frequency during water adsorption: influence of microcantilever
K Lakshmoji1, K Prabakar1, S Tripura Sundari1
1Materials Science Group, Indira Gandhi Centre for Atomic Research, Kalpakkam ,603102, Tamil Nadu, India.
Abstract:
This paper reports the effect of dimensions of microcantilever (MC) on its resonance frequency and bending upon adsorption of water molecules. Study is conducted on three MCs having the dimensions of 450 × 40 × 2.5 μm(3) (MC1), 225 × 30 × 3 μm(3) (MC2) and 125 × 35 × 4.5 μm(3) (MC3). The measured resonant frequency showed the expected negative shift in MC1, initially positive followed by a negative shift in MC2 and only positive shift in MC3 during adsorption. This behavior is attributed to changes in the stiffness of the MC associated with the surface stress. The surface stress generated on the MC has been derived from its bending measurements upon water adsorption. The change in the stiffness of MC evaluated from an independent estimate of expected frequency shift showed that the relative stiffness change of MC increases linearly with the surface stress scaled with cube of width to height ratio of MCs, confirming the dimensional dependence of adsorption induced stiffness change.
More Related Videos
11:34Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
Published on: December 20, 2013
08:58Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
Published on: December 2, 2022