Quantification of V(D)J recombination by real-time quantitative PCR
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Updated: Apr 25, 2026

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification
Published on: October 11, 2024
Fatima-Zohra Braikia1, Guillaume Chemin1, Mohammed Moutahir1
1CNRS UMR 5089; IPBS (Institut de Pharmacologie et de Biologie Structurale); FRBT, 205 route de Narbonne, BP 64182, F-31077 Toulouse, France; Université de Toulouse; UPS; IPBS F-31077 Toulouse, France.
Abstract:
B and T lymphocytes have the unique capacity to somatically rearrange their antigen receptor loci through V(D)J recombination. D-JH and VH-DJH recombination events are usually visualized by semi-quantitative PCR followed by detection of end products, which is time consuming and requires the use of hazardous elements. Additionally, it necessitates relatively large amounts of genomic DNA which could be limiting when the cell populations of interest are rare. Here, we describe a real-time quantitative PCR assay for a fast quantification of V(D)J recombination events at the IgH locus.
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