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Quantification of V(D)J recombination by real-time quantitative PCR

Fatima-Zohra Braikia1, Guillaume Chemin1, Mohammed Moutahir1

  • 1CNRS UMR 5089; IPBS (Institut de Pharmacologie et de Biologie Structurale); FRBT, 205 route de Narbonne, BP 64182, F-31077 Toulouse, France; Université de Toulouse; UPS; IPBS F-31077 Toulouse, France.

Immunology Letters
|August 17, 2014
PubMed
Summary

This study introduces a rapid, real-time quantitative PCR assay for measuring V(D)J recombination in B and T lymphocytes. This method offers a faster and more efficient alternative to traditional techniques for analyzing antigen receptor gene rearrangement.

Keywords:
B lymphocyteIgH locusReal-time quantitative PCRV(D)J recombination

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