Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid
Yonggang Lv1, Xiaoying Hao, Yongqiang Sha
1Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, P. R., China, yglv@cqu.edu.cn.
Abstract:
Improper fluid shear stress (FSS) can cause serious damages to bone marrow mesenchymal stem cells (MSCs). Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptide for 24 h. Then, the effects of MGF E peptide on the viability, proliferation and cell apoptosis of MSCs were investigated. MGF E peptide pretreatment could recover the cellular metabolic activity of MSCs reduced by 72 dyne cm(-2) FSS and had a synergistic effect with FSS on the cellular metabolic viability of MSCs under 24 and 72 dyne cm(-2) FSS. These results suggested that MGF E peptide pretreatment could be an effective method for the protection of FSS damage in bone tissue engineering.
Insights
Mechano-growth factor (MGF) E peptide protects bone marrow mesenchymal stem cells (MSCs) from fluid shear stress (FSS) damage. Pretreatment with MGF E peptide enhances MSC viability and proliferation, proving beneficial for bone tissue engineering applications.
Area of Science:
- Biomedical Engineering
- Stem Cell Biology
- Mechanobiology
Background:
- Fluid shear stress (FSS) poses a significant threat to the integrity and function of bone marrow mesenchymal stem cells (MSCs).
- Understanding the cellular responses to mechanical stimuli is crucial for advancing regenerative medicine and tissue engineering.
- Mesenchymal stem cells are vital for bone regeneration, making their protection under mechanical stress a key research area.
Purpose of the Study:
- To investigate the protective effects of Mechano-Growth Factor (MGF) E peptide pretreatment against fluid shear stress (FSS)-induced damage in bone marrow mesenchymal stem cells (MSCs).
- To evaluate the impact of MGF E peptide on MSC viability, proliferation, and apoptosis under varying FSS conditions.
- To determine the potential of MGF E peptide as a protective agent in bone tissue engineering.
Main Methods:
- Bone marrow mesenchymal stem cells (MSCs) were pretreated with MGF E peptide for 24 hours.
- MSCs were subsequently exposed to controlled levels of fluid shear stress (FSS) for 30 minutes.
- Cellular metabolic activity, viability, proliferation, and apoptosis were assessed to quantify the effects of MGF E peptide and FSS.
Main Results:
- MGF E peptide pretreatment effectively restored cellular metabolic activity in MSCs damaged by 72 dyne/cm² FSS.
- A synergistic protective effect was observed between MGF E peptide pretreatment and FSS at 24 and 72 dyne/cm² levels, enhancing cellular metabolic viability.
- The study demonstrated that MGF E peptide positively influences MSCs' response to mechanical stress, mitigating FSS-induced detrimental effects.
Conclusions:
- MGF E peptide pretreatment serves as an effective strategy to protect bone marrow mesenchymal stem cells (MSCs) from damage induced by fluid shear stress (FSS).
- This protective mechanism holds significant promise for improving the outcomes of bone tissue engineering applications by preserving MSC function under mechanical loading.
- Further research into MGF E peptide's role in mechanotransduction pathways could unlock new therapeutic avenues for bone regeneration.
