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[Effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action]
Objective:
To establish a biofilm model of Haemophilus influenzae and observe the effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action.
Method:
Thirty strains of Haemophilus influenzae were isolated from adenoids of children with adenoidal hypertrophy. Two strains which could build stronger biofilms was selected in a 96-well plate. The effect of ambroxol on biofilms were determined by crystal violet, and the structure of biofilms were observed by scanning electron microscope (SEM). The numbers of viable bacterial in biofilm after ambroxol treatmented determined by plate culture count.
Result:
Through crystal violet assay, significant difference (P < 0.01) between the two group after treatment was found when ambroxol concentration reached at 0.25 mg/ml and 0.49 mg/ml. The biofilms was destroyed by SEM. Ambroxol had the positive effect on bacterial killing by plate culture count,and the effect was in a dose dependent.
Conclusion:
Ambroxol could destroy the biofilm of Haemophilus influenzae, and had bactericidal function in vitro.
Insights
Ambroxol effectively disrupts Haemophilus influenzae biofilms and demonstrates bactericidal activity in vitro. This study highlights ambroxol
Area of Science:
- Microbiology
- Pharmacology
- Medical Research
Background:
- Adenoidal hypertrophy in children is often associated with Haemophilus influenzae.
- Bacterial biofilms contribute to persistent infections and antibiotic resistance.
- Understanding treatments that target biofilms is crucial for managing chronic infections.
Purpose of the Study:
- To develop a reliable in vitro biofilm model for Haemophilus influenzae.
- To investigate the efficacy of ambroxol in eradicating Haemophilus influenzae biofilms.
- To assess the bactericidal effects of ambroxol on Haemophilus influenzae.
Main Methods:
- Isolation of Haemophilus influenzae strains from children with adenoidal hypertrophy.
- Establishment of a 96-well plate biofilm model using selected potent biofilm-forming strains.
- Quantification of biofilm biomass using crystal violet assay.
- Microscopic structural analysis of biofilms via scanning electron microscopy (SEM).
- Determination of viable bacterial counts post-ambroxol treatment using plate culture.
Main Results:
- Ambroxol demonstrated significant biofilm disruption at concentrations of 0.25 mg/ml and 0.49 mg/ml (P < 0.01).
- SEM imaging confirmed the destructive effect of ambroxol on biofilm architecture.
- Plate culture counts indicated a dose-dependent bactericidal effect of ambroxol against Haemophilus influenzae in biofilms.
Conclusions:
- Ambroxol possesses the capability to effectively destroy Haemophilus influenzae biofilms.
- Ambroxol exhibits significant in vitro bactericidal activity against Haemophilus influenzae.
- These findings suggest a potential therapeutic role for ambroxol in managing infections caused by Haemophilus influenzae biofilms.
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