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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Electrophoresis: Overview01:20

Electrophoresis: Overview

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Updated: Apr 25, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
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Recent progress in analytical capillary isotachophoresis.

Zdena Malá1, Petr Gebauer, Petr Boček

  • 1Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic.

Electrophoresis
|August 19, 2014
PubMed
Summary

This review surveys analytical capillary isotachophoresis (ITP) research from 2012-2014, categorizing papers by nature, techniques, and instrumentation. It highlights ITP

Keywords:
Analytical electrophoresisIsotachophoresis (ITP)

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary isotachophoresis (ITP) is a powerful separation technique.
  • Recent advancements have expanded its applications and capabilities.

Purpose of the Study:

  • To provide a comprehensive survey of analytical capillary ITP literature published between 2012 and early 2014.
  • To categorize and analyze trends in ITP techniques, instrumentation, and applications.

Main Methods:

  • Literature review and categorization.
  • Analysis of papers based on theoretical, technical, and instrumental aspects.
  • Examination of applications in microfluidic and hyphenated systems.

Main Results:

  • The review covers theoretical studies, instrumentation, single-column and column-switching applications.
  • Specific focus on ITP in microfluidic systems, on-line ITP-Capillary Zone Electrophoresis (CZE), and transient ITP (tITP).

Conclusions:

  • Analytical capillary ITP holds a significant position among contemporary separation techniques.
  • The review identifies potential future trends and research directions in the field.