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Updated: Apr 25, 2026

Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

Published on: April 22, 2019

Sequential gene silencing using wavelength-selective caged morpholino oligonucleotides.

Sayumi Yamazoe¹, Qingyang Liu, Lindsey E McQuade

¹Departments of Chemical and Systems Biology and Developmental Biology, Stanford University School of Medicine, 269 Campus Drive, CCSR 3155, Stanford, CA 94305 (USA) http://chen.stanford.edu.

Angewandte Chemie (International Ed. in English)

|August 19, 2014

Summary

Researchers developed spectrally differentiated caged morpholino oligonucleotides (cMOs) for sequential gene inactivation. This method, demonstrated in zebrafish, offers a precise reverse-genetic tool for studying gene function and developmental mechanisms.

Keywords:

antisense agents cage compounds developmental biology gene expression oligonucleotides

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Area of Science:

Developmental Biology
Molecular Biology
Genetics

Background:

Sequential gene inactivation is crucial for understanding complex biological processes.
Existing reverse-genetic tools may lack precision for temporal control of gene function.
Zebrafish embryos are a powerful model system for studying early development.

Purpose of the Study:

To introduce and validate spectrally differentiated caged morpholino oligonucleotides (cMOs) for precise, sequential gene inactivation.
To demonstrate the efficacy of wavelength-selective illumination for controlling cMO activity.
To apply this novel technique to investigate the mechanisms of mesoderm patterning in zebrafish.

Main Methods:

Design and synthesis of spectrally differentiated cMOs.
Application of wavelength-selective illumination for spatiotemporal control of cMOs.
Utilizing zebrafish embryos as a model organism for reverse-genetic studies.

Main Results:

Demonstrated successful sequential inactivation of gene function using cMOs and selective light.
Confirmed the efficacy of this approach in zebrafish embryos.
Provided insights into the mechanisms governing mesoderm patterning through targeted gene manipulation.

Conclusions:

Spectrally differentiated cMOs represent a significant advancement in reverse-genetic methodologies.
This technique enables precise temporal control over gene function, facilitating detailed mechanistic studies.
The developed probes and illumination strategy are valuable tools for dissecting developmental pathways.