Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SIRT1 rs10823108 and FOXO1 rs17446614 responsible for genetic susceptibility to diabetic nephropathy.

Scientific reports·2017

Same author

Repetitive Transcranial Magnetic Stimulation Ameliorates Cognitive Impairment by Enhancing Neurogenesis and Suppressing Apoptosis in the Hippocampus in Rats with Ischemic Stroke.

Frontiers in physiology·2017

Same author

GSK-3Beta-Dependent Activation of GEF-H1/ROCK Signaling Promotes LPS-Induced Lung Vascular Endothelial Barrier Dysfunction and Acute Lung Injury.

Frontiers in cellular and infection microbiology·2017

Same author

Decreased high density lipoprotein cholesterol is an independent predictor for persistent organ failure, pancreatic necrosis and mortality in acute pancreatitis.

Scientific reports·2017

Same author

Pancreatic neuroendocrine neoplasms: Correlation between MR features and pathological tumor grades.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban·2017

Same author

Two new monoterpenoid indole alkaloids from Alstonia rostrata.

Natural product research·2017

Search research articles

Related Experiment Video

Updated: Apr 25, 2026

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Published on: October 20, 2023

[Comparison and analysis between CLL-hBMSC and N-hBMSC].

Huan Wang¹, Jun Zhou¹, Jing-Jing Xu¹

¹Central Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi

|August 19, 2014

Summary

Human bone marrow stromal cells from chronic lymphocytic leukemia (CLL-hBMSC) patients and healthy donors exhibit similar characteristics. These cells do not statistically differ in their ability to support CLL cell survival, suggesting comparable microenvironments.

More Related Videos

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Published on: April 12, 2015

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Published on: July 11, 2025

Related Experiment Videos

Last Updated: Apr 25, 2026

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Published on: October 20, 2023

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Published on: April 12, 2015

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Published on: July 11, 2025

Area of Science:

Hematology
Oncology
Cell Biology

Background:

Chronic lymphocytic leukemia (CLL) microenvironment plays a crucial role in disease progression.
Human bone marrow stromal cells (hBMSC) are key components of this microenvironment.
Understanding the differences and similarities between CLL-hBMSC and normal hBMSC (N-hBMSC) is vital for modeling the CLL niche.

Purpose of the Study:

To compare and analyze CLL-hBMSC and N-hBMSC.
To provide theoretical evidence for establishing a CLL-hBMSC interaction model.
To investigate the role of LTβR-NF-κB signaling in CLL-hBMSC and N-hBMSC.

Main Methods:

Isolation and culture of hBMSC from CLL patients and healthy donors.
Analysis of mRNA expression for adhesion molecules (VCAM-1, ICAM-1) and LTβR using real-time PCR.
Determination of LTβR and NF-κB family member protein expression via Western blot.
Assessment of CLL cell survival using flow cytometry.

Main Results:

Successful establishment of CLL-hBMSC, showing similarities to N-hBMSC.
Comparable mRNA expression of adhesion molecules and LTβR between CLL-hBMSC and N-hBMSC.
Similar protein expression levels of NF-κB family members and similar activation patterns upon LTα1β2 stimulation.
No significant difference in the capacity of CLL-hBMSC and N-hBMSC to protect CLL cell survival.

Conclusions:

There is no statistical difference in the efficiency of generating hBMSC from healthy donors versus CLL patients.
LTβR-NF-κB signaling molecules are expressed and activated similarly in both CLL-hBMSC and N-hBMSC.
The findings suggest that hBMSC from CLL patients and healthy donors create comparable microenvironments for CLL cells.