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Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
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Lymphocytes subsets reference values in childhood.

F Tosato1, G Bucciol, G Pantano

  • 1Department of Laboratory Medicine, University Hospital of Padova, Padova, Italy.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|August 19, 2014
PubMed
Summary
This summary is machine-generated.

This study establishes age-matched reference values for lymphocyte subsets and activation markers in children. These findings provide essential guidelines for diagnosing primary immunodeficiency diseases in pediatric patients.

Keywords:
lymphocyte subsetspediatric populationprimary immunodeficienciesreference values

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Area of Science:

  • Immunology
  • Pediatrics
  • Clinical Diagnostics

Background:

  • Immunophenotyping is crucial for diagnosing primary immunodeficiency diseases.
  • Interpreting immunophenotyping data requires reliable, age-matched reference values, especially for pediatric populations.
  • Current reference values for pediatric lymphocyte subsets and activation markers are often lacking.

Purpose of the Study:

  • To analyze the distribution of lymphocyte subsets and activation markers in children aged 0-18 years.
  • To establish age-matched reference values using percentiles for pediatric immunophenotyping.
  • To provide a valuable guideline for interpreting pediatric immunophenotype data.

Main Methods:

  • Blood samples were collected from children aged 0-18 years referred to the University Hospital of Padova.
  • Lymphocyte subsets and activation markers were analyzed using immunophenotyping techniques.
  • Age-matched reference values were calculated and expressed as percentiles.

Main Results:

  • The study successfully analyzed lymphocyte subset and activation marker distributions across the pediatric age range.
  • Age-specific percentile reference values were generated for key lymphocyte populations.
  • These data offer a foundation for improved diagnostic accuracy in pediatric primary immunodeficiencies.

Conclusions:

  • The established age-matched reference values are vital for accurate interpretation of pediatric immunophenotyping.
  • This study addresses a critical need for reliable reference data in diagnosing primary immunodeficiency diseases in children.
  • The findings will serve as a practical guideline for clinicians and researchers in the field.