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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Published on: February 8, 2015
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Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic
Chen Sun1, Mingxing Ouyang, Zhenning Cao
1School of Biomedical Engineering and Sciences, Virginia Tech-Wake Forest University, Blacksburg, Virginia 24061, USA.
Chemical Communications (Cambridge, England)
|August 19, 2014
Abstract:
Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ∼100 cells with high spatiotemporal resolution.
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