Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synovial Fluid Mesenchymal Stem Cells for Knee Arthritis and Cartilage Defects: A Review of the Literature.

The journal of knee surgery·2020

Same author

A framework for assessing carbon effect of land consolidation with life cycle assessment: A case study in China.

Journal of environmental management·2020

Same author

Precise control of the interlayer twist angle in large scale MoS<sub>2</sub> homostructures.

Nature communications·2020

Same author

Atomic-Precision Repair of a Few-Layer 2H-MoTe<sub>2</sub> Thin Film by Phase Transition and Recrystallization Induced by a Heterophase Interface.

Advanced materials (Deerfield Beach, Fla.)·2020

Same author

A single molecular sensor for selective and differential colorimetric/ratiometric detection of Cu<sup>2+</sup> and Pd<sup>2+</sup> in 100% aqueous solution.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2020

Same author

Effect of extracellular polymer substances on the tetracycline removal during coagulation process.

Bioresource technology·2020

Search research articles

Related Experiment Video

Updated: Apr 25, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

Tumor bioengineering using a transglutaminase crosslinked hydrogel.

Josephine Y Fang¹, Shih-Jye Tan¹, Zhi Yang¹

¹Nimni-Cordoba Tissue Engineering and Drug Discovery Laboratory, Division of Plastic and Reconstructive Surgery, Departments of Surgery and Biomedical Engineering, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.

|August 19, 2014

Summary

This summary is machine-generated.

Researchers developed a novel 3D culture system using transglutaminase-crosslinked gelatin gel (Col-Tgel) to better model the tumor microenvironment for cancer research and drug development.

More Related Videos

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Published on: June 16, 2022

Author Spotlight: Patient-Informed 3D Model for Studying Glioblastoma Invasion via Interstitial Fluid Flow

Author Spotlight: Patient-Informed 3D Model for Studying Glioblastoma Invasion via Interstitial Fluid Flow

Published on: October 18, 2024

Related Experiment Videos

Last Updated: Apr 25, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Published on: June 16, 2022

Author Spotlight: Patient-Informed 3D Model for Studying Glioblastoma Invasion via Interstitial Fluid Flow

Author Spotlight: Patient-Informed 3D Model for Studying Glioblastoma Invasion via Interstitial Fluid Flow

Published on: October 18, 2024

Area of Science:

Biotechnology
Cancer Research
Drug Development

Background:

Developing physiologically relevant 3D models for cancer research is challenging.
Existing models often fail to accurately mimic the complex tumor microenvironment.

Purpose of the Study:

To present a novel 3D culture system, Col-Tgel, for mimicking the tumor microenvironment.
To highlight the advantages of Col-Tgel over existing alternatives for cancer research and drug development.

Main Methods:

Utilized a transglutaminase-crosslinked gelatin gel (Col-Tgel) system.
Incorporated collagen-derived peptides for cell-matrix interactions.
Enabled control over gel stiffness and utilized transparent properties for imaging.
Developed as a cure-in-situ delivery vehicle for in vivo tumor xenograft formation.

Main Results:

The Col-Tgel system mimics key aspects of the tumor microenvironment, including cell-matrix interactions, multicellular spheroids, and metabolic gradients.
The system offers controllable gel stiffness and transparency for imaging-based screening.
Col-Tgel facilitates in situ tumor xenograft formation with enhanced tumor cell uptake.

Conclusions:

The Col-Tgel 3D system provides a unique platform for accurately modeling in vitro and in vivo tumor formation and progression.
This advanced model system holds significant potential for improving cancer research and accelerating drug development.