Effect of substrate age on microleakage of dentine adhesive agents

The present investigation was performed to determine the effect of substrate age on the microleakage of two new dentine adhesive agents. Class 5 preparations were placed at the cemento-enamel junctions of two groups of extracted human molar teeth which had been stored in water for less than 3 months or for approximately 18 months. Silux Plus was used to restore cavities that had been primed with either Scotchbond 2 or GLUMA. The restored teeth were thermally stressed for 100 temperature cycles and placed in a dye solution for 24 h. Leakage was measured under a microscope at X50 magnification. Under the experimental conditions, there was no significant difference in microleakage which could be attributed to the two different post-extraction tooth storage periods.